MHC Class I Dextramer For Staining Of Primary Human T Cells

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Immudex

Product Name:

HLA-B*2705 MHC class I Dextramer

Catalog Number:

WJ3334

 

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We use this dextramer from Immudex to stain primary human CD8 T cells that have been transduced with lentiviral TCR construct. We find staining with dextramers to be much easier and consistent than using the traditional tetramers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Transduced human CD8 T cells

Primary Incubation

1:5 dilution, 10min, room temperature,

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry, PE

Results Summary

Very good dextramer staining on cells transduced with the specific TCR, low background

Additional Notes

None

Related Categories

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Summary

The Good

Great staining, low background

The Bad

Expensive

The Bottom Line

Best MHC class I multimer for flow cytometry.

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