Cell Signaling Technology
p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
#9107
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Antibody that works well, even on low amounts of protein (1µg), without nonspecific binding.
Western Blot
HCT-116 lysate in several concentrations
1:2000 in 5% BSA -TBS-T, O/N at 4°C
5% BSA
1:2500 Anti-mouse in 5% BSA -TBS-T, 1h at RT
N/A
ECL westernsure with 30sec exposure time
The MAPK (ERK1/ERK2) works very well, even in high dilutions (1:2000) up to 1µg of protein as input. The signal is strong and there is no aspecific binding.
p44/42 MAPK (Erk1/2) (3A7) Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/2). It does not cross-react with either JNK/SAPK or p38 MAP kinase.
Works well on low amounts of protein, works well in higher dilutions
None
A good antibody that works well against MAPK.