Human CD56 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human CD56

Catalog Number:

561903

Our lab is interested in in enhancing the anti-HIV activity if antibodies by improving their NK-cell mediated ADCC activity. As such, we set up many in vitro ADCC assays with purified NK cells via CD56 positive selection and HIV infected target cells to study antibody ADCC capacity. We need good antibodies that can detect the quality of our purification, as well as distinguish the effector cells from target cells in these assays.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified CD56+ Cells from human PBMC

Primary Incubation

1;10 dilution; 30 min at 4 degrees celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good staining of cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works great.

The Bad

High amount required for staining of 1 sample

The Bottom Line

Good antibody with good staining and high specificity.

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