Anti human CD8a for FACS

October 18, 2016

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

APC-Cyanine7 Anti-Human CD8a (RPA-T8)

Catalog Number:

25-0088

We use anti human CD8 antibodies frequently to stain human PBMCs for flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD14 depleted PBMCs

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS APC-Cy7 channel

Results Summary

Antibody works well, giving us a clear population of CD8 positive cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works well at a reasonable dilution

The Bad

None

The Bottom Line

Good antibody to use for staining of human PBMCs

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