Fluidigm/DVS Sciences
Anti-Human CD69-162Dy
3162001B
Browse Similar Products: CD69 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. Recently we've incorporated mass cytometry into our technique to study more features per cell. We chose CD69-162Dy because it was commercially available.
Flow Cytometry
Human PBMC
0.1uL CD69-162Dy in 100uL PBS with 10^6 cells; 25 minutes; room temperature
None.
CyTOF (Fluidigm)
We titrated CD69-162Dy in tandem with a fluorescently labeled CD69 and chose 0.1uL as the optimal volume to use. It worked to detect low levels of CD69+ on T cells ex vivo, and especially after overnight stimulation with SEB (1ug/mL).
CD69-162Dy works great @ 0.1uL!
CD69-162Dy is a great option for anyone building a mass cytometry panel that is interested in lymphocyte activation.