Fluidigm
Anti-Human CD86-156Gd
3156008B
Browse Similar Products: CD86 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We recently have begun employing mass cytometry to extend the number of parameters we can measure per cell. We chose CD86-156Gd to measure B cell activation because it was commercially available.
Flow Cytometry
Human PBMC
0.5uL CD86-156Gd in 100uL PBS with 10^6 cells; 25 minutes; room temperature
None.
CyTOF (Fluidigm)
We used a fluorescently-labeled version of CD86 to determine the optimal volume of CD86-156Gd to use in our mass cytometry panel. 0.5uL was the optimal volume and staining on unstimulated and SEB stimulated (1ug/mL) B cells is shown in the attached image.
CD86-156Gd works great!
CD86-156Gd is a great option for studying B cell activation in a mass cytometry panel.