BioLegend
Alexa Fluor® 647 anti-human CD160 Antibody
341203
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Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We chose to incorporate CD160-AF467 into one of our panels largely based on channel availability.
Flow Cytometry
Human PBMC
0.5uL CD160-AF647 in 200uL PBS with 10^6 cells; 15 minutes; room temperature
None.
BD LSRFortessa
First, we titrated CD160-AF647 alone to determine which volume yielded the best stain index. 0.5uL worked the best and when added into a multi-color panel looked great without any bleedover issues. At 0.5uL/sample our staining pattern looked similar to the TDS and had higher intensity than pictured in BioLegend's figure (cost-effective!!).
0.5uL CD160-AF647 works great!
CD160-AF647 is a great option to place CD160 in a multi-color panel.