1uL Anti-Human CD20-APC From BD Works Great

Rheumatology
Vanderbilt University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD20 APC Clone L27

Catalog Number:

340940

Our group studies lymphocytes in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. Occasionally we also study B cells, and we used CD20-APC in a multi-color panel studying B cell activation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1uL CD20-APC in 200uL PBS with 10^6 cells; 15 minutes; room temperature

Blocking Agent

None.

Secondary Incubation

None.

Tertiary Incubation

None.

Detection

BD LSRFortessa

Results Summary

1uL CD20-APC works well to detect CD20+ B cells. The attached image shows CD20 expression on all live cells (black) and on live CD19+ cells (yellow/green). As expected when cells were stimulated overnight with SEB (1ug/mL) CD20 expression was downregulated, as evidenced by a shift in CD20 MFI.

Additional Notes

None.

Related Categories

Image Gallery

Summary

The Good

CD20-APC works great to detect CD20+ B cells!

The Bad

Expensive.

The Bottom Line

1uL CD20-APC works well to detect CD20+ cells in multi-color panels.

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