BD Biosciences
PE Mouse Anti-Human CD69
555531
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Browse Similar Products: CD69 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We included CD69-PE in one of our smaller panels to study T cell activation in response to superantigen and antigen stimulation.
Flow Cytometry
Human PBMC
2uL CD69-PE in 200uL PBS with 10^6 cells; 15 minutes; room temperature
None.
BD LSRFortessa
2uL CD69-PE worked well to detect CD69+ T cells. As shown in the image there was little CD69 expression ex vivo but there was a dramatic increase CD69+ T cells after stimulation in vitro, as expected.
2uL CD69-PE works well to detect activated T cells.
CD69-PE is a great option for studying T cell activation by flow cytometry.