eBiosciences
Anti-Human CD223 (LAG-3) PerCP-eFluor® 710
46-2239
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Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We picked Lag-3-PCPeF710 because the channel was open in a panel of 13 colors focused on inhibitory receptor expression on lymphocytes.
Flow Cytometry
Human PBMC
1uL Lag-3-PCPeF710 in 200uL PBS with 10^6 cells; 15 minutes; room temperature
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BD LSRII Flow Cytometer
1uL Lag-3-PCPeF710 worked great in a 13-color panel to detect Lag-3+ cells. As expected, Lag-3 expression was low without stimulation but higher after overnight stimulation with SEB (1ug/mL). Of note, we had CD3 on the AF700 channel and repeated FMOs indicated there was no bleedover between the two channels at the titrated volumes chosen for each antibody.
1uL Lag-3-PCPeF710 works great; good option for big multi-color panel!
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Lag-3-PCPeF710 works great to detect Lag-3+ lymphocytes in human PBMC.