BD Biosciences
FITC Mouse Anti-Human CD57
555619
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Browse Similar Products: CD57 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype.
Flow Cytometry
Human PBMC
5uL CD57-FITC in 200uL PBS with 10^6 cells; 15 minutes; room temperature
None
BD LSRFortessa
I used an expired vial of CD57-FITC that our lab purchased years ago and did a single color stain with volumes ranging from 1-20uL/sample and 5uL yielded the best stain index. The frequency of CD57+ cells was comparable to a newer (non-expired) version of CD57 on a different fluorochrome so we have been using up the remaining CD57-FITC and it works great.
Probably with newer vials you could titrate down and use even less volume to be the most cost-efficient.
Great staining pattern!
None.
This antibody works great to detect CD57+ cells in human PBMC samples.