BD Biosciences
Alexa Fluor® 700 Mouse anti-Human CD197 (CCR7)
561143
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Browse Similar Products: CD197 Flow Cytometry Antibodies
This antibody was used in a pediatric study involving healthy and asthmatic patients. Multiple phenotyping panels were used to screen these patients for any changes in cell populations. T cells are part of one of these panels. This antibody works well to separate the Naive cells from Central Memory, effectors or EMRA T cells .
Flow Cytometry
Human PBMC from whole blood
5/100 dilution in final volume of 50ul (PBS+ 2%FBS) for 15min at RT at dark
FcR Block (Miltenyi) for 3min at RT
None
LSRII
Nice separation of TEM, EM, CM or naive CD4+ T cells or CD8+ T cells with the addition of CD45RA BV570 antibody (Biolegend cat#304131).
Nice separation
none
Separation is always donor dependent.