Enzo
COMET SCGE Assay Kit
ADI-900-166
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The general aim of the research is to study the function of a gene in DNA damage repairs in cancer cells. The specific goals of the experiment were to examine a role of a gene in DNA damage repair in MDA-MB-231 triple negative breast cancer cell lines. Cells after scramble (left) or gene-specific (right) shRNA knock-down were subjected to Enzo Comet assay. This kit makes the complicated comet assay easy and handy to handle.
Single Cell Electrophoresis Assay
MDA-MB-231 triple negative breast cancer cell lines
Cells were collected and rinsed twice with ice cold PBS and combined with LMAgarose at 37˚C at a ratio of 1: 10 (v/v) and immediately pipetted onto slides. For cell lysis, the slides were immersed in in prechilled Lysis Solution at 4˚C, for 30 to 60 minutes, and then in freshly prepared Alkaline Solution, pH>13, for 20 to 60 minutes at room temperature, in the dark. The slides were subjected to electrophoresis at 15 V for 25 minutes (0.6 V/cm), and stained in CYGREEN® Nucleic Acid Dye for 30 minutes. Images were taken with a fluorescence microscope and analyzed with the CometScore software.
Resuspend cells at 2 x 105 cells/ml but not 1 x 105 cells/ml in ice cold 1X PBS (Ca2+ and Mg2+ free). It will give the signals from more cells when taking images.
The results are excellent. It works all the times.
None
Easy to use
Long procedures
Easy, simple and works all the time
Product Name: Comet SCGE assay kitSupplier Page