5uL CD19-BV711 Works Well To Detect Human B Cells

October 14, 2016

Rheumatology
Vanderbilt University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV711 Mouse Anti-Human CD19

Catalog Number:

563036

Our group studies lymphocytes in the blood of untreated chronic HIV+ individuals. We have included this antibody conjugate in panels to characterize plasmablast populations in human PBMC samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL CD19-BV711 in 200uL PBS with 10^6 cells; 15 minutes; room temperature

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRFortessa

Results Summary

5uL CD19-BV711 provided the best stain index value of the titrations between 0.5-5uL. Human PBMC were stained ex vivo yielding the results shown in the figure.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well to detect B cells in human PBMC

The Bad

Expensive and you have to use 5uL per sample

The Bottom Line

Good alternative if the BV711 channel fits well in a multi-color panel but there are cheaper options if there are other empty channels.

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