CD45 PE Antibody for Flow Cytometry

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

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Quality of Results

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Company:

BD Pharmingen

Product Name:

Human CD45 PE antibody

Catalog Number:

552833

The CD45 antibody was used to detect hematopoietic progenitors from in vitro differentiation. We generated blood progenitors from in vitro culture using non-human primate iPSCs. The blood progenitors would be typically CD34CD45 double positive cells. The clone of CD45 used, was reported to cross-react with NHP cells. Hence we used this antibody for our detection of progenitors. 

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Hematopoietic progenitors derived from NHP iPSCs

Primary Incubation

5ul of antibody for 100ul of cells in FACS buffer for 20 mins at RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

MAQSQuant

Results Summary

The antibody gave a good separation of the populations.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good seperation

The Bad

None

The Bottom Line

This antibody could be used to detect HP.

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