CD8a Antibody For Flow Cytometry

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD8a Antibody

Catalog Number:

100722

I am interested in studying the trafficking of immune cells between the colon and mesenteric lymph nodes in colon cancer. Therefore, as a first step we had used a panel of antibodies to check the frequency of different types of immune cells in the lymph nodes of mice with colon cancer.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse mesenteric lymph node cells

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

CD8a antibody positive cells are clearly distinguished with the antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very clear staining with no background problem

The Bad

None

The Bottom Line

Highly recommended

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