Stimulation of cultured mouse cells using TGF-ß1

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Company:

R&D Systems

Product Name:

TGF-β1

Catalog Number:

7666-MB-005

The aim of this experiment was to stimulate TGF-Beta 1 during the directed differentiation protocol & test the expression levels of a set of genes. The main reasons were the stability of the TGF-Beta 1 in our cultures and it was recommended by colleagues.

Experimental Design and Results Summary

Application

In vitro studies

Starting Material

Culture mouse embryonic stem cells

Protocol Overview

TGF-β1 (10 ng/ml ) was added to the mouse embryonic stem cells in the specification stage of the directed differentiation protocol.

Tips

Store aliquots at -80 degrees Celsius.

Results Summary

TGF-β1 impact was measured by mRNA expression analysis.

Additional Notes

None

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Summary

The Good

Stable.

The Bad

None

The Bottom Line

Stable and easy to use.

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