Antifade Mounting Medium With Nuclear Stain

University of Colorado, Denver
School of Pharmacy
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Company:

Vector Laboratories

Product Name:

VECTASHIELD Antifade Mounting Medium with DAPI

Catalog Number:

H-1200

Mounting can be used to mount the fluorescently labeled sections on the glass slide. It protects the fluorescence for long time and prevents bleaching. It specifically stains the nucleus.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Rat retina

Protocol Overview

Add one drop of the medium over section after the completion of immunostaining. Gently mount the cover slip and observe under fluorescent microscope with DAPI filter.

Tips

Use very little drop. Excess use will overflow the slide and section.

Results Summary

Can distinctly see the nucleus of each cell from the section.

Additional Notes

None

Image Gallery

Summary

The Good

Stains very fast

The Bad

Not helpful in fixing the cover slip to slide.

The Bottom Line

Can be used to visualize the nucleus from the tissue sample. However, not good as a mounting medium.

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