Good Antibody To Stain For CD161 Human Leukocytes (PE)

Pulmonary
Harvard Medical School
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD161 Antibody

Catalog Number:

339903

The aim of this project was to identify CD161+ cells in human PBMCS. The marker was used to identify Th1 and Th17 cells based on surface markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

200.000 human PBMCs were stained with the antibody for 30 minutes at room temperature in the dark. Then cells were washed with PBS twice and then taken directly to the flow cytometer

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

This is a good antibody to identify CD161+ human PBMCS

Additional Notes

None

Related Categories

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Summary

The Good

Reliable Staining

The Bad

Nothing

The Bottom Line

Good antibody to identify CD161+ human cells in PE channel

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