VWR
VWR® Disposable Pellet Mixers and Cordless Motor
47747-370
The focus of our research is to evaluate novel imaging agents, but we first need to pick a biomarker and determine its expression level in the disease model. We extract protein from cells and tissue for Western blots. We have found that large homogenizers are costly and the small handheld ones function just as well as the larger ones in most cases.
Protein Extraction
Animal tissue
Add 1mL RIPA buffer to 1.5 microcentrifuge tube. Add small amount of tissue and allow to mix for 30 min at 4 degree Celcius under constant agitation. Next, mix using the handheld homogenizer.
Don't mix too fast because the liquid may jump out of the tube.
We test the protein concentration after using this instrument and we have always recovered excellent protein concentration.
Handheld and cordless
None
Low price, small, convenient
No drawbacks of this product
This is an excellent product to have in any lab that is conducting Western blots.