Good Inexpensive CD8 Antibody

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Tonbo

Product Name:

PerCPCy5.5 antihuman CD8

Catalog Number:

65-0088

Antibody works great and isn't expensive. Use at 1:50. Some issues compensating this color with APC but not a huge problem.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

30 min on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Fortessa

Results Summary

Image shows T cells isolated from human spleen. CD8 is on y axis, CD4 on x axis.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use, good separation

The Bad

Color bleeds into other channels

The Bottom Line

Great CD8 antibody

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