Biolegend
Anti-human CD3 BV510
317332
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Browse Similar Products: CD3 Flow Cytometry Antibodies
I use this antibody for identification of T cells. This antibody can be used for a variety of experiments, for example identifying the number/percentage of T cells within a given population, and also for cell sorting. For cell sorting, I first gate on CD3+ cells to identify T cells and then can use antibodies against a variety of T cell markers (CD4, CD8, CD45RA, etc.) to sort different T cell subset. I use antibodies against CD3 with a variety of different fluorochromes and this is one of many colors that I have available so that I have flexibility when designing flow panels. The use of this antibody requires that your flow cytometer have a violet laser so it is not suitable for many models. Also note spillover into other brilliant violet colors when selecting panel. For more simple panels I would recommend choosing less expensive fluorochromes (eg FITC) and these would also be suitable for less advanced flow cytometers. This might be an antibody you select if you are designing panels with several colors.
Flow Cytometry
Human splenocytes
30 min on ice
None
BD Fortessa
See two images. First shows just the CD3 antibody. In the second, you can also see it vs CD4. antibody used at 1:50
Very good separation
Price
Great CD3 antibody