CD4 Antibody That Works, Nothing Special Though

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD4 BV711

Catalog Number:

317440

This antibody gives clear separation but is not as bright as I would expect from a BV711 color. Easy to use, works well at 1:50

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

30 min on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Fortessa

Results Summary

See two images. Both human splenocytes. One shows CD3 on x axis and CD4 on y axis. Second image is showing CD4 and CD8, already prior gates for CD3 positive cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Antibody works to separate CD4 populations

The Bad

Not so bright

The Bottom Line

Decent antibody

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