Good Antibody To Identify CD45RA+ Human PBMCs

Pulmonary
Harvard Medical School
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Company:

Biolgend

Product Name:

APC anti-human CD45RA Antibody

Catalog Number:

304111

This antibody was used for an immune phenotypic analysis of PBMCs of children with arthritis. Due to a limited amount of sample we had to use reliable antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Peripheral Blood

Primary Incubation

50ul of peripheral blood was stained with the CD45RA antibody for 30 minutes. After the incubation period cells were lysed with BD lysing buffer and washed twice with PBS. After washing, cells were directly taken to the flow cytometer.

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

This is a good antibody to identify CD45RA+ human cells.

Additional Notes

None

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Summary

The Good

Good staining of CD45RA + human PBMCs

The Bad

Nothing

The Bottom Line

This is a good antibody to identify CD45RA+ cells

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