Excellent Antibody To Identify Human CD28+ PBMCs

Pulmonary
Harvard Medical School
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Purified anti-human CD28 Antibody

Catalog Number:

302901

This antibody was used for an immune phenotypic analysis of peripheral blood cells in children with arthritis. Due to the limited amount of sample we needed antibodies with a reliable staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

Peripheral blood was incubated with CD28 antibody for 30 minutes. After that cells were lysed with BD lysing solution and then washed twice with PBS. After the washing steps, cells were immediately taken to the flow cytometer

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

This is a good antibody to identify human CD28+ PBMCs. We have never had a problem with it.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable staining of CD28+ cells

The Bad

Nothing

The Bottom Line

This is a reliable antibody to stain CD28+ human PBMCs.

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