Bosterbio
Anti-HMOX1 PicobandTM Antibody
PB9212
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Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.
Western Blot
Whole cell extract of pancreatic tumor cell MIA PaACa2
200 ng/ml Anti-HO-1/HMOX1 Antibody (PB9212) at room temperature for 3 hours
1x PBS +0.2% Tween-20 + 10% nonfat dry milk
HRP-conjugated anti rabbit antibodies at 4oC for 12 hours
N/A
Enhanced chemiluminescence (Thermo Fisher) for one minute
A major band with 32 kD was detected
None
The signal was very strong and clean
A weak band with 80 kD was also detected
A very good antibody