Revvity
IVIS Spectrum In Vivo Imaging System
124262
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The overall goal of this research is to study where in the animal tissue the virus can go. The specific objective is to use luciferase reporter as a guide to hone in on a small pieces of the tissue that contain the infection. This product was chosen due to the availability within the department.
In vivo imaging
Rhesus macaque tissue
Tissue was washed well in PBS and imaged in IVIS to determine the background level of luminescence within the tissue. Tissue was next soaked in luciferin for 10 minutes on ice and was than incubated at 37 degrees C for 10 minutes prior to imaging in IVIS. The tissue containing foci of luminecence that were not present when tissue was imaged in PBS were excised and further studied.
The shift from cold to 37 degrees is likely beneficial
The tissue imaged pre luciferin contained minimal to no background luminecense. When tissue was exposed to luciferin foci of the infection were evident.
Dark chamber
None
Works great
Substrate is very expensive
This is a great system to study reporter expression in cells and in tissues