Good HSV Thymidine Kinase Ab

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

HSV-1 Thymidine Kinase Antikörper (vL-20)

Catalog Number:

sc-28038

This is a good HSV Thymidine Kinase antibody. It gives you 2 bands, where the lower one is the specific HSV Thymidine kinase, whereas the upper one should be the endogenous thymidine kinase. I used this as a control for viral infection.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human glioma cell lines after viral infection

Primary Incubation

5% milk, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker

Secondary Incubation

5% milk, 1h, RT, shaker

Tertiary Incubation

N/A

Detection

Bio RAD Imager

Results Summary

It is a good HSV Thymidine Kinase Ab. The lower band is the specific one. Upper is endogenous thymidine Kinase. Sometimes it gives you a lot of background. I wash my blots for 1-2hrs after the secondary Ab.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Relatively easy to use

The Bad

Sometimes gives you a lot of background

The Bottom Line

Good HSV thymidine kinase ab

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