Nice Phosphor JNK antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-SAPK/JNK (Thr183/Tyr185) Antibody

Catalog Number:

9251

This is a very good phosphor JNK ntibody. Just follow the instructions of the manufacturer. Since I am using gradient gels the two bands are close together. I also use the total JNK ab from CST which works very well. I am interested in multiple pathways inside the cell.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates from human glioma cell lines

Primary Incubation

5% BSA, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker

Secondary Incubation

5% milk, 1h, RT, shaker

Tertiary Incubation

N/A

Detection

BIO RAD Imager

Results Summary

Very good phosphor JNK Ab. If you use gradient Gels the bands appear closer together.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use, reliable

The Bad

None

The Bottom Line

Perfect easy phosphor-JNK ab.

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