Really Good Human PDL1 Antibody

September 16, 2016

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

PD-L1 (405.9A11) Mouse mAb

Catalog Number:

29122

This is a very good human PDL1 antibody. Just follow the instructions of the manufacturer. I am interested in multiple immune modulatory cascades.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Patient-derived glioma cell lysates

Primary Incubation

5% BSA, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker

Secondary Incubation

5% milk, 1h, RT, shaker

Tertiary Incubation

N/A

Detection

BIO RAD Imager

Results Summary

Perfect PDL1 antibody. You have to use concentrated ECM for WB.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use, good specificity

The Bad

None

The Bottom Line

Perfect ab. Totally recommended

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