DC-SIGN Antibody For IF Staining

September 26, 2016

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dendritics

Product Name:

DC-SIGN/CD209

Catalog Number:

DDX0202

Browse Similar Products: CD209 Immunofluorescence Antibodies

The aim of this project is to study the role of immune cells in colon cancer. In the present experiment, DC-SIGN antibody was used to detect the presence of dendritic cells in a normal human colon tissue specimen (pilot study).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human normal colon tissue specimen

Primary Incubation

1:250 dilution in blocking buffer; over night incubation at 4 deg Celsius

Blocking Agent

1x PBS + 1% BSA + 1% goat serum

Secondary Incubation

1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti mouse Alexa dye 488; 1 hour at r/t

Tertiary Incubation

DAPI was included in the last wash step

Detection

Confocal Imaging

Results Summary

Dendritic cells could be detected in the human colon tissue specimen using this antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific signal

The Bad

No

The Bottom Line

Good for IF staining

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