Sigma
Anti-ALDH1A2 antibody
HPA010022
Browse Similar Products: ALDH1A2 Immunofluorescence Antibodies
The aim of this project is study the role of retinoic acid pathway metabolic enzymes in colon cancer. In this experiment, the expression of ALDH1A2 was tested in a normal colon cancer tissue specimen (pilot study). This antibody was previously used by a lab member.
Immunofluorescence
Human normal colon tissue specimen
1:250 dilution in blocking buffer; over night incubation at 4 deg Celsius
1x PBS + 1% BSA + 1% goat serum
1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti rabbit Alexa dye 594; 1 hour at r/t
DAPI was included in the last wash step
Confocal Imaging
ALDH1A2 was expressed in human colon tissue specimen
None
Specific signal
No
Good for IF staining