Proteintech Group Inc
beta-Catenin Antibody
51067-2-AP
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The aim of this project is to study the role of the Wnt pathway in a mouse model of colon cancer. Beta-catenin is an important regulator in the Wnt pathway and in colon cancer it's expression is elevated. In the present experiment, beta-catenin expression was checked in a normal mouse colon tissue (pilot experiment).
Immunofluorescence
Mouse colon tissue sample
1:200 dilution in blocking buffer; overnight at 4 deg Celsius
1x PBS + 1% BSA + 1% goat serum
1:200 dilution (in 1xPBS + 1% BSA) of Goat-anti rabbit Alexa dye 594; 1 hour at r/t
DAPI was included in the last wash step
Confocal Imaging
Beta-catenin expression was good enough to detect in a normal mouse colon tissue
None
Specific signal
No
Good antibody for IF staining