Santa Cruz Biotechnology
Anti-alpha Actin (1A4)
sc-32251
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Anti-alpha Actin was to stain and detect smooth muscle cells harvested and grown from primary aortic tissue isolated from mice. Anti-alpha actin (sc-32251) resulted in a strong, characteristic staining pattern indicative of actin.
Immunofluorescence
Aortic tissues from mice where treated in collagenous and expanded in cell culture for 3 days priory to cellular characterization with alpha actin.
A dilution of 1:50 anti-alpha actin in 2% DGS was incubated at room temperature for 2 hours.
10% Donor Goat Serum (DGS).
A dilution of 1:100 of anti-Mouse-594 (Alexa series) in 2% DGS was incubated at room temperature for 1 hour.
N/A
594nm excitation on an inverted microscope.
The anti-alpha actin gave a very nice staining pattern of a large density of cells isolated from the mouse aorta. The staining pattern was indicative of the cytoskeletal protein actin.
Blue: Hoechst nuclear stain, red: anti-alpha actin
The antibody gave a strong characteristic staining pattern.
None to report.
I would strongly recommend this antibody to other investigators in the field.