Rabbit anti-Green Fluorescent Protein Review

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Nordic-MUbio BV

Product Name:

Rabbit anti-Green Fluorescent Protein (GFP native)

Catalog Number:

BII-nGFPab3-100

Browse Similar Products: GFP Immunofluorescence Antibodies

The overall goal of this research was to understand protein targeting to flagellar membrane. The specific goals were to understand the requirements of protein modifications and calcium binding on flagellar targeting. This product was choose because it works in both immunofluorescence microscopy and western blot applications.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Trypanosoma cuzi

Primary Incubation

anti-GFP antibody for 1 hour at room temperature

Blocking Agent

Normal goat serum

Secondary Incubation

anti-Mouse AF 488 antibody at room temperature for 30 minutes

Tertiary Incubation

None

Detection

Immunofluorescence microscopy

Results Summary

Our protein of interest was tagged with GFP. Using anti-GFP specific antibody we were able to detect a strong signal and our protein localized as expected. This antibody was also successfully used by our lab for Western Blot applications (data not shown).

Additional Notes

None

Related Categories

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Summary

The Good

Good signal

The Bad

None

The Bottom Line

Highly recommending for detection of GFP in IF and WB applications

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