Abcam
Anti-NTF97/Importin beta antibody
ab2811
Info: View Product View Product Specs
Browse Similar Products: NTF97 Western Blot Antibodies
The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. In the present experiment, knockdown of hedgehog pathway components was performed and a marker was needed to judge loading of samples for Western Blotting. NTF97 blotting was done to check if this antibody works in 3T3 cells so that it can be used at later stages.
Western Blot
NIH-3T3 cell lysate
1:1000 dilution in blocking buffer; 16 hr at 4 deg
5% milk powder in 1xTBS-T(0.1%)
Donkey anti-mouse IgG-HRP conjugated; 1:5,000 dilution; 1 hr at room temp.
None
ECL regular
NTF-97 was expressed at good levels in 3T3 cells.
Specific signal and no cross-reactive bands were observed
Good NTF97 (Importin-beta) antibody for Western Blotting