Cell Signaling Technology
HMGB1 Antibody #3935
3935
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We used mouse lung homogenates to detect HMGB1 level.
Western Blot
Mouse lung homogenates
1:1000 in 5% milk
5% Milk in room temperature for 1 hr
1:10,000 Secondary antibody in 5% milk
N/A
ECL based detection using Bio-Rad Chemi-Doc MP
We were able to detect HMGB1 in mouse lung samples.
None
Works for mouse samples
Noted none