Santa Cruz Biotechnology
Rho A Antibody (119)
sc-179
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Protein Kinase D (PKD) is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. Like many other kinases, PKD functions are mediated via phosphorylation of different substrates. The goal of this project was to demonstrate if rhotekin was a bona fide substrate of PKD and whether this phosphorylation is important for regulating RhoA. In this experiment, RhoA levels were tested for downstream analysis of RhoA activity experiments.
Western Blot
PANC-1 cells
1:500 dilution in blocking agent; 16 hr at 4 deg
5% Milk powder in 1xTBS-T(0.1%)
Goat-anti Rabbit-IgG-HRP; 10000 dilution in blocking agent; 1 hr at room temp.
None
ECL regular
RhoA expression could be nicely detected in PANC-1 cells.
Good signal
Good RhoA antibody for Western Blotting