GST antibody for Western Blotting

Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells. In this experiment, GST and GST-ARF1 were expressed and purified from bacteria. Relative levels of these proteins in a pull down experiment were determined by Western Blotting with GST antibody. This antibody was purchased by another graduate student in the lab for his specific application and recommended me to use it for my experimental purpose.