Sigma-Aldrich
Monoclonal Anti-ß-Actin antibody produced in mouse
A5316-100UL
Browse Similar Products: Beta-Actin Western Blot Antibodies
Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells. Beta-actin antibody was used asa loading control for Western Blotting experiments. This antibody was used because many papers have cited this product.
Western Blot
HeLa cell whole cell lysate
1:5000 dilution in blocking buffer; 1 hr at room temp
5% Milk powder in 1xTBS-Tween (0.1%)
Goat anti-mouse IgG-HRP conjugated; 1:10000 dilution in blocking buffer; 1 hr at room temp;
None
ECL regular
Beta-actin antibody was used as a loading control to make sure that sample loading was uniform across lanes in a SDS-PAGE gel followed by Western Blotting. This antibody is very specific and probably incubation for 20-30 min would also be fine.
Specific signal and no background issues
Perfect antibody for loading control determination in Western Blotting experiments