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Enzyme-linked Immunosorbent assay kit for Interleukin 1 beta (IL-1B)
MBS2024496
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This kit was used for quantifying Interleukin 1 beta in plasma samples of 21 days chickens for comparative analysis of immunological response.This kit can be used in other chicken body fluids.We selected this kit by suggestion of another researcher.
In vitro quantitative measurement of IL-1beta in chicken serum, plasma, tissue, cell, or biological fluids in general
21 days chicken plasma
1 - Place reagents and samples to room temperature 2 - Add 100 uL off standards and samples in the pre determined wells. Cover the plate and incubate for 60 minutes at 37ºC 3 - Remove the liquid and DO NOT WASH 4 - Add 100 uL of Detection Reagent A. Cover the plate and incubate for 1 h at 37ºC 5 - Wash the plate 5X of 2 minutes each; 6 - Add 100 uL of Detection Reagent B. Cover the plate and incubate for 1 h at 37ºC 7 - Wash the plate 5X of 2 minutes each; 8 - Add 90 uL of Substrate solution. Incubate for 20 minutes at 37ºC protected from the light. 9 - Add 50 uL of Stop solution. 10 - Measurment of the Optical density (DO) at 450nm;11 - build a standard curve with a regression line
Its appropriate standardize the method for the specific tissue before the real experiment.
The standard curve has a detection range between 7,8 and 500 pg/mL). The curve was drawn on a arithmetical scale, and the equation for quantyfing was obtained with a linear regression. The r² was iqual to 0,9884.
Sometimes, the mininum among of IL-1beta can not be determinated
It was easy to do and to analyze.
It is very expensive, each 96-well plate $750
This kit is a good product, but it would be useful more information on samples dilution or limits of detection in different samples.
Product Name: ELISA Kit for Interleukin 1 Beta (IL1b)Supplier Page