ß-Arrestin 1/2 from Cell Signaling is a Good Antibody to Use

MOLECULAR PHARMACOLOGY
MSKCC
RESEARCH ASSOCIATE

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

β-Arrestin 1/2

Catalog Number:

#4674

I tested beta arrestin protein expression level in HEK 293T cells. I tried the #4647 in CST. It works very well and I can get two bands in the expected size. I strongly recommend you guys to use the product.

Experimental Design and Results Summary

Applications

Western Blot

Sample

hek 293T cells protein

Primary Incubation

4 degree overnight

Blocking Agent

7% non fat milk in TBST buffer

Secondary Incubation

At room temperature for 1 hour in 1/10000 TBST buffer

Tertiary Incubation

ECL Chemiluminescent Substrate in Thermo Fisher scientific company

Detection

Using V3 Western Workflow™ from Bio-Rad

Results Summary

We used V3 Western Workflow™ from Bio-Rad to collect the results of antibody detecting system and I got very strong signal of positive results. So now I refer the antibody to the other people.

Additional Notes

None

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Summary

The Good

Very good

The Bad

None

The Bottom Line

N/A

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