Droplet Digital PCR Efficiency

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad Laboratories

Product Name:

BioRad QX200 Droplet Digital PCR

Catalog Number:

186-3001

I examined the efficacy of droplet digital PCR assay and compared it with qPCR, for quantitative enumeration of bacterial pathogens in environmental samples.

Experimental Design and Results Summary

Application

Detection of bacterial pathogens in environmental samples

Starting Material

Surface water and sediments

Protocol Overview

The handling of instrument is easy.

Tips

There should be minimum time gap between droplet generation and PCR

Results Summary

QX200 droplet digital PCR system is fully amenable for the quantification of bacterial pathogens and offer robust, accurate, high-throughput, affordable and more sensitive quantitation than qPCR.

Features Summary

Highly tolerant to PCR inhibitors

Additional Notes

None

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Summary

The Good

Easy to handle and highly tolerant to PCR inhibitors

The Bad

Digital droplet PCR showed higher variability and less good precision at the higher concentrations of bacterial pathogens

The Bottom Line

QX200 droplet digital PCR system may provide an opportunity to reduce the inhibitory effects of PCR inhibitors experienced with qPCR, but the methodology need to be further optimized for complex environmental samples.

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