Cell Signaling Technology
Myc-Tag Antibody
2272
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Detection of proteins which have no available antibody are made easy by epitope tags. These tags allow the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunofluorescence. The Myc-tag antibody (#2272) was used successfully to detect myc-tagged RNF215 protein.
Western Blot
HeLa cell lysate overexpressing myc-tagged RNF215 (80ug total protein)
Primary antibody (1:1000) in dilution buffer (TBST with 5% BSA) with gentle agitation overnight at 4°C.
TBST with 5% w/v nonfat dry milk
Incubated the membrane with the Goat Anti-Rabbit IgG, H & L Chain Specific Peroxidase Conjugate (Cat No: 401315) 1:10000 diluted in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
None
Incubated the membrane with appropriate amount of Luminata Crescendo Western HRP substrate (WBLUR0100 Merckmillipore) for 5 min at room temperature. Drained the membrane of excess developing solution (without letting it to dry) wrapped in transparent plastic sheet and exposed the x-ray film for 1 min.
Antibody detects the myc-tagged RNF215 expression in the Hela cells.
This antibody can be used for immunoflurescence .
Works for both immunofluorescence and western blot
This antibody works better than many antibodies available for the myc-tag.