Cell Signaling Technology
Src (L4A1) Mouse mAb
2110S
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I have developed a model of drug resistance to an anti-cancer drug, and performed proteomic analysis to understand the molecular signling pathways that confer resistance. Vials of the antibodies were leftover in the lab from previous use by other investigators. Generally, I prefer Cell Signaling antibodies because they are well-validated and tend to perform well.
Western Blot
Ovarian cancer cell line lysates
1:1000 in 5% milk in TBS-T, O/N at 4 degrees C
5% milk in TBS-T, 1 hr, RT
1:5000 anti-Rb HRP in 5% milk in TBS-T, 30 min, RT
N/A
HRP Chemiluminescence
Single band at correct size. There was some background on the blot, but I use a digital analyzer that is likely more sensitive than film. The background did not affect my ability to evaluate the band intensity.
None
Good antibody, single band
Some background on blot
Overall a good antibody. Background could be a problem for publication, but optimizing antibody concentration may help.