BUV805 CD8 Works Well

Immunology
Columbia University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BUV805 Anti-human CD8

Catalog Number:

564912

This antihuman BUV805 from BD works well. it is quite dim, which is good for abundant marker like CD8. Works optimally at 1:25 or 1:50. At 1:100 still able to distinguish populations but beyond that cannot.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human splenocytes

Primary Incubation

25min on ice in dark, 1:50

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

Works well, easy to see positive CD8 population. Also, works for intracellular staining. It is stable after fix perm with slight dimming of signal. In the picture, BUV805 is on the Y axis.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well, little overlap with other colors

The Bad

Dim antibody, but this is good for abundant markers

The Bottom Line

Use this for little spectral overlap with other colors

Share your experience with other scientists. Write a Review! »

Join the discussion