Thermo Fisher Scientific
Lipofectamine 3000
L3000-008
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Normal lipofectamin reagents are toxic to the cell which leads to the poor viability of the cell after the transfection procedure. Lipofactamin 3000 can be used to enhance transfecting capability with a very less toxicity, which can improve the viability of the cells to a higher extent when compared to the conventional transfecting agents.
Eukaryotic gene expression
DNA
The kit can be used by following the manufacturer's protocol. Briefly, the DNA is diluted in Opti MEM and P3000 reagent is added to the diluted at 1:2 ratio. Lipofectamin 3000 reagent is diluted in OptiMEM in a separate vial Now the diluted DNA and lipofectamin 3000 are mixed and incubated 20 minutes for proper lipoplexes formation.
You can try different ratios of Lipofectamin 3000 concentrations for optimizing the transfection.
Successfully transfected cells are obtained with higher viability
None
Less toxic
We cannot use complete media while transfecting.
I recommend this kit for people who desire higher viability after transfection.
Product Name: Lipofectamine 3000 Transfection ReagentSupplier Page