The Good
Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data
The Bad
Various multi step procedures decrease inter-assay reproducibility and take longer to perform as compared to R&D & eBiosciences instant kits. The CV values of the lower standard curve points were 10-20%, which was also a bit higher than the two aforementioned kits.
The Bottom Line
I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used.