Review of CD16 Antibody

Biomedical Sciences
Creighton University
Research Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

PE-Cy™5 Mouse Anti-Human CD16 3G8 RUO

Catalog Number:

555408

I used this antibody to stain cells collected from blood samples. I was testing my cells for expression of inflammatory markers in various stages of bladder cancer. I chose this specific antibody because of the well-known company and availability.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Monocytes and neutrophils isolated from blood

Primary Incubation

Antibody cocktail at 4'C

Blocking Agent

N/A for flow

Secondary Incubation

N/A for flow

Tertiary Incubation

N/A for flow

Detection

Flow Cytometry

Results Summary

The antibodies were titrated to the optimal concentration and added in a cocktail solution to the cells. The sample was then immediately sent for flow cytometry in order to get the best results; sample was kept cool at 4'C

Additional Notes

None

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Summary

The Good

This is an easily available antibody.

The Bad

Shipping time is a few days.

The Bottom Line

Overall, I would recommend this product to other scientists.

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