Bethyl Laboratories Inc
PKD3 antibody
A300-319A
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Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells.
Western Blot
HeLa cells; whole cell extract; 100 µg was loaded for SDS-PAGE
1:1000 dilution in blocking agent for 16 hr at 4 deg C
5% Milk+0.1% Tween-20 in 1xTBS
Donkey anti-rabbit IgG-HRP conjugated
None
ECL regular (chemiluminescence)
PKD3 is expressed in HeLa cells and can be specifically knocked down by siRNA.
Good signal
Good antibody for PKD3 detection by Western Blotting